VCF - Variant Calling format -

Sample 1 - FASTQ input file 
@r1/s1
TGTTAGACAAGGTAGCTG
+
GHHGCGGFE?A8C@BABD
@r2/s1
GTTAGACAAGGTAGCTGT
+
BAB@C8A?EBAB?@BBBB
@r3/s1
GTTAGACAAGGTAGCTGTGC
+
B??EHHAC@HEB??EHHAC@
@r4/s1
TAGTAGGCAGTTAGCGC
+
B??EHHAC@HEB??EHH
@r5/s1
TAGTAGGCAGTTAGCG
+
B??EHHAC@HEB??EH
Sample 2 - FASTQ input file 
@r1/s2
TGTTAGATAAGGTAGCTG
+
B??EHHAC@HEB??EHHA
@r2/s2
ATAAGGTAGCTGTGCTAG
+
B??EHHAC@HEB??EHHA
@r3/s1
GTAGGCAGTAAGCGCCAG
+
B??EHHAC@HEB??EHHA
@r4/s1
TAGTAGGCAGTAAGCGCCAG
+
B??EHHAC@HEB??EHHAC@
@r5/s1
AGCTGTGCTACTAGGCAG
+
B??EHHAC@HEB??EHHA
@r6/s1
AGCTGTGCTACTAGGCAGTA
+
B??EHHAC@HEB??EHHAC@
FASTA input file 
>Ref
AGCATGTTAGATAAGATAGCTGTGCTAGTAGGCAGTCAGCGCCAT
Alignment 
Coor    123456789012345678901234567890123456789012345
Ref     AGCATGTTAGATAAGATAGCTGTGCTAGTAGGCAGTCAGCGCCAT

+r1/s1      TGTTAGACAAGGTAGCTG
+r2/s1       GTTAGACAAGGTAGCTGT             
+r3/s1       GTTAGACAAGGTAGCTGTGC             
+r4/s1                           TAGTAGGCAGTTAGCGC  
+r5/s1                           TAGTAGGCAGTTAGCG  

+r1/s2      TGTTAGATAAGGTAGCTG            
+r2/s2            ATAAGGTAGCTGTGCTAG
+r3/s2                             GTAGGCAGTAAGCGCCAG  
+r4/s2                           TAGTAGGCAGTAAGCGCCAG
+r5/s2                   AGCTGTGCTACTAGGCAG
+r6/s2                   AGCTGTGCTACTAGGCAGTA
In each line of a VCF file is stored an information about a variant position in the genome...

 
CHROM POS ID REF ALT QUAL FILTER INFO FORMAT s1 GENOTYPE FIELDS s2 GENOTYPE FIELDS