FastQCFastQC Report
mer. 22 mars 2017
emb_0_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameemb_0_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3295732
Sequences flagged as poor quality0
Sequence length36
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATCGTATGCCGTCTTCTGCTT48636914.757540965102745Illumina Single End Adapter 2 (95% over 21bp)
AGCGTGTAGGGATCCAAACTGTAGGCACCATCAATC540931.6413045720950612No Hit
CTGTAGGCACCATCAATTACTGTAGGCACCATCAAT307930.934329611752412No Hit
TCGTAGGCACCATCAATCGTATGCCGTCTTCTGCTT301780.915669113872123Illumina Single End Adapter 2 (95% over 21bp)
GGCCAACGTTCTCAACAATAGTGACTGTAGGCACCA226130.6861298188080827No Hit
TCAGTCTTTTTCTCTCTCCTATCTGTAGGCACCATC143340.43492614083912157No Hit
TGCTTGGACTACATATGGTTGAGGGCTGTAGGCACC142660.43286286627674825No Hit
CTGTAGGCACCATCAATCTGTAGGCACCATCAATCG118100.3583422438474973No Hit
CATTGCACTTGTCCCGGCCTATCTGTAGGCACCATC100780.30578942705292783No Hit
TCACTGGGCAAAGTGTGTCTCACTGTAGGCACCATC81980.24874595385789866No Hit
GGCGAATGTCGGTATGGTCTCTCTGTAGGCACCATC76810.2330589987292656No Hit
TATTGCACACTTCCCGGCCTTTCTGTAGGCACCATC75070.2277794432314278No Hit
CTGTAGGCACCATCAATCGTATGCCGTCTTCTTCTT62530.18973023291942426No Hit
TGACTAGACCGAACACTCGTGCTCTGTAGGCACCAT62080.1883648306355007No Hit
GCACTGGGTAAAGTTTGTCCTACTGTAGGCACCATC56130.17031117821473346No Hit
CTACATATGGTTGAGGGTTGTACTGTAGGCACCATC55930.1697043327552119No Hit
TTCAATTCCCCGTCGGGGAGCCACTGTAGGCACCAT54860.16645770954677142No Hit
GACTACATATGGTTGAGGGTTGTACTGTAGGCACCA53960.16372690497892425No Hit
CTGTAGGCACCATCAATCGTATGCCGTCTTCTGTTT52440.1591148794865602No Hit
CATATGGTTGAGGGTTGTACTGTAGGCACCATCAAT51170.15526141081859812No Hit
ACTACATATGGTTGAGGGTTGTACTGTAGGCACCAT46700.14169841479829062No Hit
ATTGTACTTCATCAGGTGCTCTCTGTAGGCACCATC46230.1402723279684149No Hit
CTGTAGGCACCATCAATCGTATGCCGTCTTTTGCTT43050.13062348516202166No Hit
TACATATGGTTGAGGGTTGTACTGTAGGCACCATCA41610.12625419785346625No Hit
TAGCCTCTACTAGGCTTTGTCTCTGTAGGCACCATC39880.12100498462860451No Hit
TACTAGAGTGCATTACTGATTTCTTTCTGTAGGCAC39150.11878999870135072No Hit
CTGTAGGCACCATCAATACTGTAGGCACCATCAATC38740.11754596550933147No Hit
ATATGGTTGAGGGTTGTACTGTAGGCACCATCAATC37310.11320702047375213No Hit
ACATATGGTTGAGGGTTGTACTGTAGGCACCATCAA36760.11153819546006775No Hit
TATGGTTGAGGGTTGTACTGTAGGCACCATCAATCG36420.11050655817888104No Hit
GCGAAAGCCGAAAATTTTCAACTGTAGGCACCATCA35400.10741164633532095No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCTT556100.029.53516430
TGACTAG28950.029.5310041
GACTAGA29150.029.4312972
CCGTCTT576200.029.31199524
CGTCTTC568150.029.2885625
GCCGTCT577700.029.24234623
TATGCCG584150.029.23010820
CCAACGT38600.029.2201543
TGCCGTC578450.029.19405622
CTTCTGC559750.029.16257328
CGTATGC586900.029.13394418
GTCTTCT573000.029.10917526
TTCTGCT564100.029.1052229
GTATGCC587800.029.0842319
GCCAACG39250.029.0419582
ATGCCGT585450.029.0324921
TCGTATG591700.029.03029617
CCGGCCT36750.029.01910214
AGCGTGT75150.028.9993691
TATCACA33900.028.9354321
Produced by FastQC (version 0.11.2)