FastQCFastQC Report
mer. 22 mars 2017
emb_0_1_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameemb_0_1_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293987
Sequences flagged as poor quality0
Sequence length17-24
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGTGTAGGGATCCAAA626744.843479880400653No Hit
GGCCAACGTTCTCAACAATAGTGA270022.086728846580375No Hit
TCAGTCTTTTTCTCTCTCCTAT168451.3017905125785652No Hit
CATTGCACTTGTCCCGGCCTAT116720.9020183355783328No Hit
GGCGAATGTCGGTATGGTCTCT91580.7077350854374889No Hit
TCACTGGGCAAAGTGTGTCTCA91320.7057257916810601No Hit
TATTGCACACTTCCCGGCCTTT86740.6703313093562764No Hit
TGACTAGACCGAACACTCGTGCT68810.5317673206917845No Hit
TTCAATTCCCCGTCGGGGAGCCA66810.5163112148731015No Hit
CTACATATGGTTGAGGGTTGTA63620.49165872609230227No Hit
GCACTGGGTAAAGTTTGTCCTA63050.48725373593397775No Hit
GACTACATATGGTTGAGGGTTGTA62830.48555356429392255No Hit
CATATGGTTGAGGGTTGTA56300.43508937879592297No Hit
ACTACATATGGTTGAGGGTTGTA52890.4087367183750687No Hit
ATTGTACTTCATCAGGTGCTCT51230.3959081505455619No Hit
TAGCCTCTACTAGGCTTTGTCT46900.36244568144811345No Hit
TACATATGGTTGAGGGTTGTA46770.36144103456989907No Hit
ATATGGTTGAGGGTTGTA42980.332151714043495No Hit
ACATATGGTTGAGGGTTGTA42070.3251191858959943No Hit
TATGGTTGAGGGTTGTA41690.3221825257904446No Hit
GCGAAAGCCGAAAATTTTCAA38870.30038941658610174No Hit
TATTGCACTTGAGACGGCCTGA37460.2894928619839303No Hit
AGGGAACGGTTGCTGATGATGTA36560.282537614365523No Hit
TGACTAGACTGGTAACCTGCTC35130.2714864987051647No Hit
TAAAGCTAGATTACCAAAGCAT31330.2421198976496673No Hit
TATTGCACATTCACCGGCCTGA30560.23616929690947436No Hit
TATCACAGTTCTTTGTCACCCT30080.23245983151299046No Hit
ACACCGCGTGTTGTTGGCCT28940.2236498511963412No Hit
TGACTAGATCCACACTCATTAA28890.22326344855087416No Hit
TATCACAGCCATTTTGACGAGTT27560.21298513818145004No Hit
AGGGAATAGTTGCTGTGCTGTA27490.21244417447779615No Hit
CCCGGGTTTCGGCACCA27090.20935295331405956No Hit
GAAAGCCGAAAATTTTCAA26630.2057980489757625No Hit
GCGTGTAGGGATCCAAA26350.2036341941611469No Hit
TGACTAGATTTCATGCTCGTCT26330.20347963310296005No Hit
CCCGGGTGTCCCCTCCA26260.20293866939930616No Hit
ATAAAGCTAGACAACCATTGAA26230.20270682781202592No Hit
TAGCACCACATGATTCGGCTT26030.20116121723015765No Hit
AAAGGAACGATCGTTGTGATATG25680.19845639871188814No Hit
TCTTTGGTGATTTTAGCTGTAT25340.19582886072271205No Hit
CGAAAGCCGAAAATTTTCAA25040.19351044484990962No Hit
TGGACGGAGAACTGATAAGGGC24560.18980097945342575No Hit
TCGTAGGCACCTGAAAT23290.1799863522585621No Hit
TGACTAGATTTCATGCTCGTCTA22620.17480855680930335No Hit
AGGGAACTTCTGCTGCTGATATA21280.16445296591078581No Hit
GAACACCGCGTGTTGTTGGCCT20830.16097534210158215No Hit
TCAGTCTTTTTCTCTCTCCTA20530.15865692622877972No Hit
CCGCGTGTTGTTGGCCT20510.1585023651705929No Hit
CATCACAGTCTGAGTTCTTGCT20480.15827052358331267No Hit
TTTGGCACGCCTGAAAAACTAC19100.14760581056842148No Hit
CTTTGGTCGTCCAGCCTTAGGTGA19010.14691028580658075No Hit
ACCGCGTGTTGTTGGCCT18770.14505555310833881No Hit
TAGCCTCTACTAGGCTTTGTCA18690.14443730887559147No Hit
TCTTTGGTATTCTAGCTGTAGA18380.14204161247369562No Hit
AAGCCGAAAATTTTCAA18260.14111424612457465No Hit
TATCACAGCCATTTTGACGAGT17590.1359364506753159No Hit
TGACTGACTCATGTAGTGTGCACT17530.13547276750075543No Hit
CCCCTTGTTGCAAACCTCACG17500.13524092591347517No Hit
CCCGGGCGGAAACACCA17490.13516364538438175No Hit
CTGTAGGTACCATCAATTA16710.12913576411509542No Hit
AGTCGCTGGTGACTATATATTT16650.12867208094053495No Hit
TGACTAGATCCACACTCATTA16360.12643094559682594No Hit
TGGACGGAGAACTGATAAGGG16310.12604454295135886No Hit
ATTGTACTTCATCAGGTGCTC15530.12001666168207255No Hit
CATTGCACTTGTCCCGGCCTA15470.11955297850751205No Hit
AAAGCCGAAAATTTTCAA15190.11738912369289646No Hit
CACCGCGTGTTGTTGGCCT15140.11700272104742937No Hit
AGTCGCTGGTGACTATATATTTT15000.11592079364012156No Hit
TGCTTGGACTACATATGGTTGAGG14990.11584351311102817No Hit
TGCTGCGGATGGGGGCAGTACT14860.11483886623281378No Hit
TATCACAGCCAGCTTTGAGGAG14560.11252045036001135No Hit
TGACTAGATTTCATGCTCGTC14440.11159308401089037No Hit
TCCCGGCCGACGCACCA13920.10757449649803283No Hit
AGTCGCTGGTGACTATATATTTTT13270.10255126210696089No Hit
GGTGAACCTGCGGAAGGATCATTA12980.10031012676325188No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTGA37650.049.00791518
CGAGTTA1350.041.4174618
AGGCCCG900.039.237618
GATTAGC455.5439214E-639.237618
GTGCACT3600.035.1503518
GTTAGGA1100.034.77877818
ACGAGTT4400.034.70335817
AGTCGAA853.6561687E-1034.62140718
ATACGGT1400.033.63222518
TCGTGCT7800.033.5592917
CGACTAT1400.031.5302118
AAATAGA751.620665E-731.39007818
ACCTCGT1159.094947E-1230.70768418
AATAGTG38650.029.81842217
TCGTCTA3800.029.6587617
AAAAACG902.4330802E-829.428218
AGGAGCG1301.8189894E-1229.42819818
ATAGCGA400.005649524329.42819818
CCTAAGA400.005649524329.42819818
TTGCGGC1400.029.42819818
Produced by FastQC (version 0.11.2)