FastQCFastQC Report
mer. 22 mars 2017
emb_6_10.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameemb_6_10.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538726
Sequences flagged as poor quality0
Sequence length36
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATCGTATGCCGTCTTCTGCTT1991267.8435404214554865Illumina Single End Adapter 2 (95% over 21bp)
TCGTAGGCACCATCAATCGTATGCCGTCTTCTGCTT1196044.7111819077757895Illumina Single End Adapter 2 (95% over 21bp)
AGCGTGTAGGGATCCAAACTGTAGGCACCATCAATC453971.7881803707844015No Hit
TCGTAGGCACCTGAAATCGTAGGCACCATCAATCGT229780.905099644467343No Hit
ATGTAGGCACCATCAATCGTATGCCGTCTTCTGCTT196950.77578281389957Illumina Single End Adapter 2 (95% over 21bp)
TAAGGCACGCGGTGAATGCCAACTGTAGGCACCATC193250.7612085746945515No Hit
TGACTAGACCGAACACTCGTGCTCTGTAGGCACCAT134550.5299902391987162No Hit
CTGTAGGCACCATCAATCTGTAGGCACCATCAATCG132020.5200246107693387No Hit
AGCGTGTAGGGATCCAAACTGTAGGCACCATCACTC119230.4696450109228014No Hit
TGGAATGTAAAGAAGTATGGAGCTGTAGGCACCATC95660.37680316820326415No Hit
TAAGGCACGCGGTGAATGCCAACTGTAGGCACCCTC90240.3554538772596964No Hit
TGGTTGAGGGTTGTACTGTAGGCACCATCAATCGTA82380.32449346640795423No Hit
ACGTAGGCACCATCAATCGTATGCCGTCTTCTGCTT77460.3051136672488485Illumina Single End Adapter 2 (95% over 21bp)
TGCTTGGACTACATATGGTTGAGGGTTGTACTGTAG75180.29613278471170185No Hit
ACATATGGTTGAGGGTTGTACTGTAGGCACCATCAA64410.25370993167439104No Hit
TATCACAGCCAGCTTTGATGAGCTCTGTAGGCACCA61230.24118396392521288No Hit
CTGTAGGCACCATCAATTACTGTAGGCACCATCAAT60040.2364965734781934No Hit
TATCACAGCCAGCTTTGAGGAGCGCTGTAGGCACCA56360.2220011139445533No Hit
CTGTAGGCACCATCAATCGTATGCCGTCTTTTGCTT55380.21814091004700784No Hit
GGCCAACGTTCTCAACAATAGTGACTGTAGGCACCC54980.21656531661943826No Hit
TCGTAGGCACCATCAATCGTATGCCGTCTTTTGCTT52850.20817528161763024No Hit
AAAGGAACGATCGTTGTGATATGCTGTAGGCACCAT52420.20648151868299297No Hit
ATAAAGCTAGACAACCATTGAACTGTAGGCACCATC51630.20336972166354306No Hit
TATGGTTGAGGGTTGTACTGTAGGCACCATCAATCG48910.19265568635606994No Hit
TCTTTGGTTATCTAGCTGTATGACTGTAGGCACCAT47590.18745622804509032No Hit
GGCCAACGTTCTCAACAATAGTGACTGTAGGCACCA47400.1867078211669948No Hit
TCACAGCCAGCTTTGATGAGCTACTGTAGGCACCAT44400.17489087046022297No Hit
CATCACAGTCTGAGTTCTTGCTCTGTAGGCACCATC42730.16831276790011998No Hit
TGACTAGACTGGTAACCTGCTCCTGTAGGCACCATC42560.16764314069340291No Hit
TAAGGCACGCGGTGAATGCCACTGTAGGCACCATCA39470.15547168146542795No Hit
TACATATGGTTGAGGGTTGTACTGTAGGCACCATCA37850.14909052808377116No Hit
TAGGAACTTCATACCGTGCTCTCTGTAGGCACCATC37140.14629384974983514No Hit
TGACTAGATTTCATGCTCGTCTACTGTAGGCACCAT33870.13341337347945387No Hit
ACTACATATGGTTGAGGGTTGTACTGTAGGCACCAT33770.1330194751225615No Hit
TCACTGGGCAAAGTGTGTCTCACTGTAGGCACCATC33510.13199533939464125No Hit
GACTACATATGGTTGAGGGTTGTACTGTAGGCACCA33400.13156205120205963No Hit
CATATGGTTGAGGGTTGTACTGTAGGCACCATCAAT33130.13049852563845016No Hit
TATCACAGTTCTTTGTCACCCTCTGTAGGCACCATC33030.13010462728155775No Hit
TGGACGGAGAACTGATAAGGGCCTGTAGGCACCATC32500.12801696599002807No Hit
ATATGGTTGAGGGTTGTACTGTAGGCACCATCAATC32110.12648076239814773No Hit
TGCTTGGACTACATATGGTTGAGGGCTGTAGGCACC31940.12581113519143067No Hit
TCAGTCTTTTTCTCTCTCCTATCTGTAGGCACCATC31720.1249445588062674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31130.12262055850060227No Hit
AAAGGAACGATCGTTGTGATATCTGTAGGCACCATC30760.1211631345801004No Hit
CATTGCACTTGTCCCGGCCTATCTGTAGGCACCATC30700.12092679556596497No Hit
TGAGTATTACATCAGGTACTGGTCTGTAGGCACCAT30190.11891791394581376No Hit
ATCGTAGGCACCATCAATCGTATGCCGTCTTCTGCT28000.11029153992987034No Hit
GTCCCTGTTCGGGCGCCACTGTAGGCACCATCAATC27980.11021276025849186No Hit
GGCCAACGTTCTCAACAATAGTGACTGTAGGCGCCC27520.10840082781678685No Hit
GGTTGAGGGTTGTACTGTAGGCACCATCAATCGTAT27480.10824326847402989No Hit
TCTGTAGGCACCATCAATCGTATGCCGTCTTCTGCT26490.1043436747407952No Hit
AGCGAGGTATAGAGTTCCTACGCTGTAGGCACCATC26250.10339831868425343No Hit
TGACTAGACCGAACACTCGTGCTCTGTAGGCACCCT26040.10257113213477942No Hit
TAATACTGTCAGGTAAAGATGTCCTGTAGGCACCAT25570.10071980985738516No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG50850.029.7151811
GACTAGA56750.029.68692
ACTAGAC40300.029.6163433
AGCGTGT85450.029.4775981
CGGTGAA60400.029.46245210
ACGCGGT64100.029.4368087
ACTAGAT15650.029.3766463
TCTGCTT385100.029.37488630
CCAACGT24450.029.3382913
GTGAATG60200.029.32537512
TATCACA97000.029.31781
CGCGGTG64150.029.2527548
ATCACAG116500.029.2052272
TAGACCG27700.029.1939035
ACACTCG28250.029.17689113
TGAATGC60350.029.15315813
TAAGGCA61650.029.1491811
GCGGTGA62250.029.0921829
CTAGACC27850.029.0904354
AGACCGA27700.028.9776526
Produced by FastQC (version 0.11.2)