FastQCFastQC Report
mer. 22 mars 2017
kc167_r1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamekc167_r1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7244636
Sequences flagged as poor quality0
Sequence length36
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATCATTTTGAAAGCTGATCTGTAGGCACCATC132614218.305157084496724No Hit
TGGACGGAGAACTGATAAGGGCCTGTAGGCACCATC86060211.87916135469056No Hit
TGAGATCATTTTGAAAGCTGATTCTGTAGGCACCAT3964965.472959580025829No Hit
CTGTAGGCACCATCAATCGTATGCCGTCTTCTGCTT2371763.27381527519119Illumina Single End Adapter 2 (95% over 21bp)
TCACAGCCAGCTTTGATGAGCTACTGTAGGCACCAT1568752.165395197219018No Hit
TATCACAGCCAGCTTTGATGAGCTCTGTAGGCACCA1370791.8921447537184752No Hit
TCACAGCCAGCTTTGATGAGCTCTGTAGGCACCATC1267891.750108632096906No Hit
TGGACGGAGAACTGATAAGGGCTGTAGGCACCATCA977381.3491084990329396No Hit
TATCACAGCCAGCTTTGAGGAGCGCTGTAGGCACCA591260.8161348617100984No Hit
TGGACGGAGAACTGATAAGGGCCTGTAGGCACCCTC531130.7331355226128683No Hit
TAGCTGCCTTGTGAAGGGCTTCTGTAGGCACCATCA439940.6072630840252016No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370850.5118959737935764No Hit
TGAGATCATTTTGAAAGCTGATCTGTAGGCACCCTC338330.46700759016740107No Hit
TAATACTGTCAGGTAAAGATGTCCTGTAGGCACCAT321970.4444253651943314No Hit
TATCACAGCCAGCTTTGAGGAGCCTGTAGGCACCAT321270.4434591330744567No Hit
CTGTAGGCACCATCAATCGTATGCCGTCTTCTTCTT310100.4280408291044574No Hit
TATCACAGCCAGCTTTGAGGAGCTGTAGGCACCATC310040.42795800920846816No Hit
CATCACAGTCTGAGTTCTTGCTCTGTAGGCACCATC279960.38643763468585585No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN272790.37654065711514006No Hit
TGAGATCATTTTGAAAGCTGATTTCTGTAGGCACCA260850.3600594978132787No Hit
TCTTTGGTGATTTTAGCTGTATGCTGTAGGCACCAT235140.32457117238188365No Hit
AGCGAGGTATAGAGTTCCTACGCTGTAGGCACCATC222220.30673728811219775No Hit
CATCACAGTCTGAGTTCTTGCCTGTAGGCACCATCA178350.24618214082805542No Hit
TATCACAGCCATTTTGACGAGTTCTGTAGGCACCAT168760.2329447607857731No Hit
TGAGATCATTTTGAAAGCTGATCTGTATGCACCATC166090.2292592754142513No Hit
TAGGAACTTCATACCGTGCTCTCTGTAGGCACCATC136520.18844287000754767No Hit
TGGACGGAGAACTGATAAGGGCCTGTAGGCAACATC136200.18800116389560498No Hit
TGAGATCATTTTGAAAGCTGACTGTAGGCACCATCA135610.18718676825171063No Hit
TGAGATCATTTTGAAAGCTGATTCTGTATGCACCAT135500.18703493177573033No Hit
TGAGATCATTTTGAAAGATGATCTGTAGGCACCATC131300.1812375390564826No Hit
TATCACAGCCATTTTGACGAGTCTGTAGGCACCATC130060.17952592787270472No Hit
TATCACAGCCAGCTTTGATGAGCCTGTAGGCACCAT128730.17769008684494295No Hit
AAATTGACTCTAGTAGGGAGTCTGTAGGCACCATCA122360.16889737455408388No Hit
TGGACGGAGAACTGATAAGGGCCTGTAGGCACCATA114490.1580341648634935No Hit
TATCACAGCCAGCTTTGAGGAGCGTCTGTAGGCACC107890.14892397630467563No Hit
TATCACAGCCAGCTTTGATGAGCTTCTGTAGGCACC102760.14184287519759448No Hit
TGAGATCATTTTGAAAGCTGATCTGTAGGCACCATA101830.14055916680976105No Hit
GTGCATTGTAGTCGCATTGTCCTGTAGGCACCATCA101700.14037972370178434No Hit
TGCTTGGACTACATATGGTTGAGGGCTGTAGGCACC97330.13434767461056704No Hit
TGAGATCATTTTGAAAGCTGATCTGTAGGCAACATC88000.12146918078423817No Hit
TCACAGCCAGCTTTGATGAGCTTCTGTAGGCACCAT79760.11009524840171404No Hit
TGGACGGAGAACTGATAAGGGCTGTAGGCACCCTCA77090.10640976303019226No Hit
TGAGATCATTTTGAAAGCTGATTCTGTAGGCACCCT74540.102889917450649No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGATC1612100.029.94750417
GAAAGCT2182350.029.94456113
AAGCTGA2193000.029.9442215
GATCATT2231950.029.938074
ATCATTT2234150.029.937475
GAGATCA2222300.029.9371742
TATCACA479550.029.9367731
AGATCAT2224700.029.929823
CTGATCT1626250.029.9290518
TCATTTT2251850.029.9254476
TTGAAAG2235750.029.91674411
AAAGCTG2196000.029.90894914
TGAGATC2234850.029.9002061
TGAAAGC2188100.029.89192812
TGGACGG1341900.029.8882081
AGCTGAT2191450.029.88335816
ATAAGGG1295900.029.8672815
TTTGAAA2257300.029.85793310
TAAGGGC1269400.029.8559916
ACTGATA1329900.029.83713511
Produced by FastQC (version 0.11.2)