FastQCFastQC Report
mer. 22 mars 2017
ml-DmD32_r1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameml-DmD32_r1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6382837
Sequences flagged as poor quality0
Sequence length36
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATCATTTTGAAAGCTGATTCTGTAGGCACCAT88003213.787474127883886No Hit
TGGACGGAGAACTGATAAGGGCCTGTAGGCACCATC3281025.140378800210628No Hit
TGAGATCATTTTGAAAGCTGATCTGTAGGCACCATC2553364.000352821167139No Hit
TAATACTGTCAGGTAAAGATGTCCTGTAGGCACCAT1310742.053538262061212No Hit
TATCACAGCCAGCTTTGATGAGCTCTGTAGGCACCA1278992.0037954909392175No Hit
TGGACGGAGAACTGATAAGGGCTGTAGGCACCATCA1201741.8827678037211353No Hit
TCACAGCCAGCTTTGATGAGCTCTGTAGGCACCATC1133121.77526075003952No Hit
TATCACAGCCAGCTTTGAGGAGCTGTAGGCACCATC925041.4492615117697663No Hit
TATCACAGCCATTTTGACGAGTTCTGTAGGCACCAT766321.200594657203372No Hit
TCACAGCCAGCTTTGATGAGCTACTGTAGGCACCAT727901.1404019873921267No Hit
CTGTAGGCACCATCAATCGTATGCCGTCTTCTGCTT661541.0364356789935258Illumina Single End Adapter 2 (95% over 21bp)
CATCACAGTCTGAGTTCTTGCCTGTAGGCACCATCA653211.0233850558928577No Hit
GTGCATTGTAGTCGCATTGTCCTGTAGGCACCATCA619080.9699135353135291No Hit
TAGCCTCTACTAGGCTTTGTCTCTGTAGGCACCATC590520.9251685418255237No Hit
CATCACAGTCTGAGTTCTTGCTCTGTAGGCACCATC557730.873796401192761No Hit
TATCACAGCCAGCTTTGAGGAGCGCTGTAGGCACCA541190.8478831591657441No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA465510.7293151932283403No Hit
TAGGAACTTCATACCGTGCTCTCTGTAGGCACCATC436530.6839121851302172No Hit
TATCACAGCCAGCTTTGAGGAGCCTGTAGGCACCAT374560.5868236961087993No Hit
TCAGTCTTTTTCTCTCTCCTATCTGTAGGCACCATC358250.5612707954158943No Hit
TAATACTGTCAGGTAAAGATGTCTGTAGGCACCATC340800.5339318550669553No Hit
TGAGATCATTTTGAAAGCTGATTCTGTATGCACCAT328030.5139250775164712No Hit
ATTGTACTTCATCAGGTGCTCCTGTAGGCACCATCA272110.42631513228365375No Hit
TGAGATCATTTTGAAAGCTGATTCTGTAGGCACCCT272010.4261584621383877No Hit
TGGACGGAGAACTGATAAGGGCCTGTAGGCACCCTC264920.41505054883901943No Hit
CTAAGTACTAGTGCCGCAGGAGCTGTAGGCACCATC261920.41035044448103564No Hit
TATCACAGCCATTTTGACGAGTCTGTAGGCACCATC244830.3835755166550548No Hit
CGTACGGTTTAAACTTCGACTGTAGGCACCATCAAT224520.35175581015150476No Hit
TGAGATCATTTTGAAAGCTGACTGTAGGCACCATCA191770.3004463375768487No Hit
TCTTTGGTGATTTTAGCTGTATGCTGTAGGCACCAT181040.2836356309897934No Hit
TGACTAGATTTCATGCTCGTCTCTGTAGGCACCATC174880.27398475004140005No Hit
TGACTAGATCCACACTCATTCTGTAGGCACCATCAA163160.25562300901621016No Hit
TCAGGTACCTGAAGTAGCGCGCTGTAGGCACCATCA143010.22405397474508593No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN135710.21261705414065876No Hit
TATCACAGCCAGCTTTGATGAGCTGTAGGCACCATC133890.20976565749681528No Hit
TCAGGTACCTGAAGTAGCGCGCGCTGTAGGCACCAT133300.20884130363974512No Hit
TGGCAGTGTGGTTAGCTGGTTCTGTAGGCACCATCA131580.20614657714116777No Hit
CTGTAGGCACCATCAATCGTATGCCGTCTTCTTCTT131440.20592723893779522No Hit
TGAGATCATTTTGAAAGCTGATTTCTGTAGGCACCA121090.18971187890275124No Hit
TGCTTGGACTACATATGGTTGAGGGCTGTAGGCACC116810.18300639668536106No Hit
CATCACAGTCTGAGTTCTTGCTGTAGGCACCATCAA114200.1789173058939152No Hit
TAGCTGCCTTGTGAAGGGCTTCTGTAGGCACCATCA112540.17631658148249751No Hit
TAGCACCATGAGATTCAGCTCCTGTAGGCACCATCA109670.1718201483133597No Hit
TGGACGGAGAACTGATAAGGCTGTAGGCACCATCAA106200.16638369427262517No Hit
TAAAGCTAGATTACCAAAGCATCTGTAGGCACCATC104560.16381430389026072No Hit
TCTTTGGTATTCTAGCTGTAGACTGTAGGCACCATC103780.16259227675718493No Hit
TCTTTGGTGATTTTAGCTGTATCTGTAGGCACCATC102790.1610412423190503No Hit
TATCACAGCCAGCTTTGAGGACTGTAGGCACCATCA101690.1593178707211229No Hit
TCAGGTACCTGAAGTAGCGCGCCTGTAGGCACCATC101420.15889486132890437No Hit
TGGCAGTGTGGTTAGCTGGTTGTGCTGTAGGCACCA100420.15732815987624313No Hit
TAGCACCACATGATTCGGCTTCTGTAGGCACCATCA93550.1465649208964603No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN91190.14286750546817975No Hit
TAATACTGTCAGGTAAAGATGTCCTGTAGGCACCCT89240.13981243763549028No Hit
TGGCAGTGTGGTTAGCTGGTTGCTGTAGGCACCATC88570.13876274766220725No Hit
ATTGTACTTCATCAGGTGCTCTGGCTGTAGGCACCA87880.137681723659871No Hit
TGGACGGAGAACTGATAAGGGCTCTGTAGGCACCAT87130.13650669757037506No Hit
TGGACGGAGAACTGATAAGGGCTGTAGGCACCCTCA84840.13291895124378078No Hit
TATCACAGCCAGCTTTGATGAGCTCTGTAGGCACCC84010.13161858903807194No Hit
TAGCCTCTACTAGGCTTTGTCTCTGTAGGCACCCTC82010.12848518613274945No Hit
ATTGTACTTCATCAGGTGCTCTCTGTAGGCACCATC79830.1250697769659479No Hit
GTGCATTGTAGTCGCATTGTCTGTAGGCACCATCAA78280.12264138971432295No Hit
TATCACAGCCAGCTTTGATGAGCCTGTAGGCACCAT77660.12167003481367297No Hit
TGAGATCATTTTGAAAGCTGATCTGTAGGCACCCTC76520.11988399515763914No Hit
TGAACACAGCTGGTGGTATCCCTGTAGGCACCATCA73590.11529355990134167No Hit
TCAGTCTTTTTCTCTCTCCTACTGTAGGCACCATCA71120.11142380731326837No Hit
TGGACGGAGAACTGATAAGGGCTGTAGGCACCATCC70410.11031144928187889No Hit
TCTCGAATAGCGTTGTGACTGACTGTAGGCACCATC69600.10904242110522328No Hit
TATCACAGCCAGCTTTGAGGAGCTGTAGGCACCCTC68800.10778905994309428No Hit
TAATACTGTCAGGTAAAGATGCTGTAGGCACCATCA68080.10666103489717818No Hit
TGAGATCATTTTGAAAGCTGATTCTGTAGTCACCAT67910.10639469565022575No Hit
TGAGATCATTTTGAAAGCTGATTCTGTAGGCAACAT67160.10521966956072981No Hit
AAGATGGAGTAGTTTTTTCTGTAGGCACCATCAATC67040.10503166538641047No Hit
AGCGAGGTATAGAGTTCCTACGCTGTAGGCACCATC65870.1031986246867968No Hit
TCAGTCTTTTTCTCTCTCCTCTGTAGGCACCATCAA64160.10051956520274605No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAGCT1468000.029.99966413
TTGAAAG1485500.029.99571411
TGAAAGC1469350.029.99291812
TACGCCG200.005168627529.97665220
CGCCTAG900.029.9633271
AAAGCTG1470000.029.95963114
TTTGAAA1496750.029.9569910
GAGATCA1495400.029.945172
AGATCAT1499900.029.9144923
GTAAAGA242000.029.90649613
TATCACA617500.029.90511
ACTGATA668450.029.90347311
AAGCTGA1463100.029.90299215
GATAAGG659350.029.89548314
GTCGCAT124000.029.89478911
AGGTAAA243650.029.88536811
CTGATTC1122450.029.88433618
GATCATT1509750.029.8842094
ATCATTT1515550.029.8800645
GAACTGA659950.029.8757449
Produced by FastQC (version 0.11.2)