FastQCFastQC Report
mer. 22 mars 2017
ml-DmD32_r2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameml-DmD32_r2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7947259
Sequences flagged as poor quality0
Sequence length36
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGACGGAGAACTGATAAGGGCCTGTAGGCACCATC6052027.615229351402792No Hit
TGAGATCATTTTGAAAGCTGATCTGTAGGCACCATC3091873.890486015367059No Hit
CTGTAGGCACCATCAATCGTATGCCGTCTTCTGCTT1905642.3978581797824883Illumina Single End Adapter 2 (95% over 21bp)
CTGTAGGCACCATCAATCGTATGCCGTCTTCTTCTT1261471.5873019867604667No Hit
TGGACGGAGAACTGATAAGGGCCTGTAGGCACCCTC1120261.4096180834171883No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1113951.40167823900039No Hit
TCACAGCCAGCTTTGATGAGCTACTGTAGGCACCAT1020221.2837382045809758No Hit
TGAGATCATTTTGAAAGCTGATTCTGTAGGCACCAT983491.2375210119614826No Hit
TCACAGCCAGCTTTGATGAGCTCTGTAGGCACCATC852401.0725710587763655No Hit
TATCACAGCCAGCTTTGATGAGCTCTGTAGGCACCA691100.8696079994372903No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN515010.6480347500943408No Hit
TGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN482150.6066871609444212No Hit
TATCACAGCCAGCTTTGAGGAGCGCTGTAGGCACCA461670.5809172697152565No Hit
TGGACGGAGAACTGATAAGGGCTGTAGGCACCATCA391770.49296241635008997No Hit
TATCACAGCCAGCTTTGAGGAGCTGTAGGCACCATC378930.4768059025130551No Hit
TGAGATCATTTTGAAAGCTGATCTGTATGCACCATC370860.4666514580687505No Hit
TAATACTGTCAGGTAAAGATGTCCTGTAGGCACCAT309390.38930403551715126No Hit
TATCACAGCCAGCTTTGAGGAGCCTGTAGGCACCAT308740.38848614346153815No Hit
CATCACAGTCTGAGTTCTTGCTCTGTAGGCACCATC305460.38435893431936724No Hit
TGAGATCATTTTGAAAGCTGATCTGTAGGCACCCTC287030.36116854880405935No Hit
TGGACGGAGAACTGATAAGGGCCTGTATGCACCATC270160.339941104222223No Hit
TANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN237920.29937365826381146No Hit
TGAGATCATTTTGAAAGCTGATCTGTAGTCACCATC220430.27736607049046724No Hit
TAGGAACTTCATACCGTGCTCTCTGTAGGCACCATC209730.2639023089596048No Hit
TCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN186490.23465952223275977No Hit
CATCACAGTCTGAGTTCTTGCCTGTAGGCACCATCA182350.22945017898623915No Hit
CTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN177680.22357393914052634No Hit
TGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN176470.2220514016216157No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN175150.22039045160098594No Hit
TGAGATCATTTTGAAAGCTGATTCTGTATGCACCAT169310.21304200605516949No Hit
TGGACGGAGAACTGATAAGGGCCTGTAGGCCCCATC144260.18152170452730934No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN143510.18057798292467883No Hit
TGAGATCATTTTTAAAGCTGATCTGTAGGCACCATC143200.18018791132892487No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA139320.17530572490464952No Hit
TCACAGCCAGCTTTGATGAGCTACTGTAGGCACCCT137580.17311629078654664No Hit
TGGACGGAGAACTGATAAGGGCCTGTAGTCACCATC136020.17115334985307512No Hit
TATCACAGCCAGCTTTGATGAGCTCTGTAGGCACCC124550.1567207008101787No Hit
TGAGATCATTTTGAAAGCTGATCTTTAGGCACCATC122600.1542670246433393No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN115960.14591194272138355No Hit
TCACAGCCAGCTTTGATGAGCTCTGTAGGCACCCTC115430.14524504612219133No Hit
TGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN113230.1424767960878084No Hit
TGGACGGAGAACTGATAAGGGCCTTTAGGCACCATC112260.14125624948173957No Hit
TGGACGGAGAACTGATAAGGGCCTGTCGGCACCATC111560.1403754426526177No Hit
TCACAGCCAGCTTTGATGAGCTACTGTATGCACCAT110260.13873965854139142No Hit
CTGTAGGCACCATCAATCGTATTCCGTCTTCTTCTT110060.13848799944735662No Hit
TGGACGGAGAACTGATAATGGCCTGTAGGCACCATC106310.1337693914342039No Hit
TCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN105360.13257401073753858No Hit
TGAGATCATTTTGAAAGCTGATTCTTTAGGCACCAT103680.13046007434764614No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN102490.12896270273813903No Hit
TGAGATCATTTTGAAAGCTGATTCTGTAGGCACCCT100240.1261315379302474No Hit
CTGTAGGCACCATCAATCGTATGCCTTCTTCTTCTT97270.12239440038383045No Hit
TGCTTGGACTACATATGGTTGAGGGCTGTAGGCACC96050.1208592799102181No Hit
CTGTAGGCACCATCCATCGTATGCCGTCTTCTGCTT94560.11898441965965875Illumina Single End Adapter 2 (95% over 21bp)
TATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN93490.1176380435065725No Hit
TGGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN93410.1175373798689586No Hit
TATCACAGCCAGCTTTGAGGAGCGCTGTAGGCACCC91820.11553669007138186No Hit
TGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN91360.11495787415510177No Hit
TGGACGGAGAACTGATAAGGGCTGTAGGCACCCTCA88190.11096907751465002No Hit
TATCACAGCCATTTTGACGAGTTCTGTAGGCACCAT87810.11049092523598388No Hit
TCACAGCCAGCTTTGATGAGCTCTGTATGCACCATC85720.1078610877033201No Hit
CTGTAGGCACCATCAATCGTATGCCGTCTTCTCCTT85060.10703061269300522No Hit
TATCACAGCCAGCTTTGATGAGCCTGTAGGCACCAT82310.10357030015002656No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCTGA835750.029.99555815
TAAGGGC1142650.029.96236816
AAGGGCC1039200.029.96083817
GCTGATC616100.029.9569317
AGGGCCT1036000.029.93653318
ATAAGGG1129800.029.93330415
GAAAGCT801150.029.90643713
GATAAGG1157450.029.88743414
AAAGCTG841100.029.8735114
AGCTGAT845150.029.8556716
CTGATCT676200.029.8551318
TTGAAAG804900.029.8497111
GGGCCTG1000400.029.81107519
TTTGAAA857750.029.77013210
CTGATAA1305250.029.75597212
ACTGATA1309100.029.75373611
TGATAAG1220250.029.7451813
TGATCTG603850.029.7386919
AGGTAAA98050.029.72523911
GATCTGT606150.029.7077120
Produced by FastQC (version 0.11.2)