FastQCFastQC Report
mar. 10 mai 2022
SRR6148374_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6148374_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15547444
Sequences flagged as poor quality0
Sequence length35-101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCGACTAACGATCACGTCGTGTTTCTTATTGGTCGCGCTAAGCGCGG194950.1253903857122753No Hit
CGAAGATACTAGAACACAGAACTCTTCAAAGAAAAAATGAAAGCAATATT170150.109439210715279No Hit
GTCGAAGATACTAGAACACAGAACTCTTCAAAGAAAAAATGAAAGCAATA169970.10932343605804273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC56350.026.964569
TGTAGGG35600.023.84854916-17
GACTAAC78100.023.3813516
ACTAACG82750.022.4546247
CTACCCG62200.021.89308294-95
CTTAACT53650.021.4134521
AGAGCGT74450.020.5933918
GGCCCGG17150.020.5482949
CTAACGA104000.020.3308898
ATACTAG101950.020.2010086
GTAGGCT44050.019.16959230-31
GATCGGA79950.018.834761
CGACTAA99300.018.8055155
CTAGAAC111700.018.1509659
ACTTCGC59550.016.9088945
TCTACCC70550.016.81071392-93
ACTAGAA124650.016.3386258
TCGACTA120000.015.6378954
CGCTTTC86500.015.5563224
TGTAGGC46550.015.48543130-31
Produced by FastQC (version 0.11.5)