FastQCFastQC Report
mer. 11 mai 2022
SRR12596365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12596365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24435947
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGT769850.31504815426224325No Hit
GAAAAAGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGT689170.28203122228084715No Hit
AAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAG640320.2620401820318239No Hit
AGACAATCTAACTTGAGAAAAAGAAAACGCAGCAATGAGTCTCTCTGATA610370.24978364865499178No Hit
TCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCATA567100.23207612948252018No Hit
GTCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCAT556250.2276359496114474No Hit
CTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGA553370.22645735808806594No Hit
GAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGC518980.21238382944602066No Hit
AGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAG510020.20871710026216703No Hit
CTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGTGCCATCA466240.19080087217409664No Hit
CCGCGATCTTCTGCAATCCAGCTGGTGTTCATCGGTGATGGGAATCTAGT460970.18864421337957557No Hit
GCCAGATCAACAAGATTTCATGGCGTTTCCTCTGTGGGAGATGCTGTGGA459740.1881408565831314No Hit
CGAGGATCTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGT376080.15390440976156972No Hit
CTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGTTCGCTCA334840.13702763391981493No Hit
GCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGCCCTATG321260.13147024750053682No Hit
CAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGCCCTATGG315030.12892072486488862No Hit
CCACGATCACATTGTGTGCGAGGATCTTGAAGTTGGCCGGGTCAATACGC307930.12601516937321888No Hit
CTGGACTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGA296300.12125578763123032No Hit
TGGGAATCTAGTGGTACTGTCTGCACAGAGCGGAAACGACGACACACAGA279770.11449116336682183No Hit
GTCAATACGCAGCTTGAAAGCATGAAGTTCGCTCAGGGAGGACAGAGCTC268920.11005098349574911No Hit
CAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATG265730.10874552969033695No Hit
GTGGCCTTGATGTTATCCATGTTCTTGATGGCTCTCTCCAGACCTCCCAT263570.1078615860478008No Hit
CTCAATCCCGATGAACTCGGACCTCAGGCCCTGGCCAGGTGTCTGATCGT256870.10511972382326742No Hit
CTTAAACAAGTGCATAAGGACACCCTAAAACGTAGACATGGTTGAGTGGA254950.10433399614101306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCTA153950.069.821714
CCGCGAT114450.063.977931
ATCTAAC159050.059.3679166
TCTAACT168300.055.591437
GGACTTA151850.052.866573
AATCTAA184900.052.6830945
GACAATC186250.051.7601552
CTAACTT200450.048.902938
GACTTAA169500.047.7002264
CGCGATC155650.047.539772
TAACTTG216450.045.3036969
CTTAAAC290000.044.4843521
AGACAAT218100.043.3541261
GCGATCT188800.039.5455673
CGCAATC95400.039.4992682
ACAATCT296200.037.84643
CCGCAAT106450.036.830321
GTGCCTA286050.032.984677
TAAACAA386150.032.759463
ACTTAAA227800.031.6998795
Produced by FastQC (version 0.11.5)