FastQCFastQC Report
mer. 11 mai 2022
SRR12596365_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12596365_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24435947
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGT751240.30743232500872586No Hit
CTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGA691310.2829069812600265No Hit
AAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAG655880.26840785012342677No Hit
GAAAAAGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGT618960.25329896156674425No Hit
AGACAATCTAACTTGAGAAAAAGAAAACGCAGCAATGAGTCTCTCTGATA598270.24483192732411801No Hit
CTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGTGCCATCA578540.23675775692261897No Hit
TCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCATA518560.21221195151552752No Hit
GTCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCAT479460.19621093465295203No Hit
GAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGC469820.19226592691496672No Hit
AGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAG464280.18999877516512864No Hit
CCGCGATCTTCTGCAATCCAGCTGGTGTTCATCGGTGATGGGAATCTAGT437880.17919501953413142No Hit
GCCAGATCAACAAGATTTCATGGCGTTTCCTCTGTGGGAGATGCTGTGGA427940.1751272418457938No Hit
CGAGGATCTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGT419530.17168559090425264No Hit
CTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGTTCGCTCA411850.16854268017523527No Hit
CTGGACTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGA345830.14152510643438537No Hit
CCACGATCACATTGTGTGCGAGGATCTTGAAGTTGGCCGGGTCAATACGC333480.13647107681155143No Hit
CAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGCCCTATGG330120.13509605336760633No Hit
CTTAAACAAGTGCATAAGGACACCCTAAAACGTAGACATGGTTGAGTGGA329510.13484642113522344No Hit
CAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATG310830.12720194555995723No Hit
CTCAATCCCGATGAACTCGGACCTCAGGCCCTGGCCAGGTGTCTGATCGT283840.11615674235993391No Hit
TGGGAATCTAGTGGTACTGTCTGCACAGAGCGGAAACGACGACACACAGA276170.11301792396259493No Hit
GCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGCCCTATG276130.11300155463588131No Hit
CACGATCACATTGTGTGCGAGGATCTTGAAGTTGGCCGGGTCAATACGCA255430.10453042806157667No Hit
CTCGGACCTCAGGCCCTGGCCAGGTGTCTGATCGTGTATCCCTGGACTCA248820.10182539682214896No Hit
GTCAATACGCAGCTTGAAAGCATGAAGTTCGCTCAGGGAGGACAGAGCTC248380.101645334228299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCTA156950.067.020094
CCGCGAT109100.064.8767851
ATCTAAC157200.059.5386436
GGACTTA166200.055.752413
TCTAACT167000.055.6991847
GACTTAA180100.052.049124
AATCTAA188600.051.3830035
GACAATC185200.051.1600572
CTTAAAC332450.049.3603861
CGCGATC147800.048.3229372
CTAACTT204300.046.763328
TAACTTG219650.043.65929
AGACAAT222050.042.1878171
GCGATCT182700.040.03793
ACAATCT284150.038.3360523
TAAACAA417100.038.061383
CGCAATC94000.036.8411642
ACTTAAA235500.035.585925
CCGCAAT101600.035.2227481
TTAAACA463700.035.199022
Produced by FastQC (version 0.11.5)