FastQCFastQC Report
mer. 11 mai 2022
SRR12596366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12596366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19997056
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGT281900.14097075089453168No Hit
GCCAGATCAACAAGATTTCATGGCGTTTCCTCTGTGGGAGATGCTGTGGA257440.12873895037349498No Hit
GAAAAAGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGT256340.1281888694015759No Hit
AAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAG238810.11942257900362932No Hit
AGACAATCTAACTTGAGAAAAAGAAAACGCAGCAATGAGTCTCTCTGATA225680.11285661249335903No Hit
CTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGA213870.10695074314939158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCTA91200.048.7084354
CCGCGAT63400.043.0810661
ATCTAAC96050.038.394496
TCTAACT98750.036.9797447
CTAACTT106250.034.2338338
AATCTAA119250.033.145755
GGACTTA96750.030.957123
GACAATC124700.030.506092
CGCGATC91150.030.2734012
CTTAAAC184000.029.1401391
TAACTTG126250.028.9918829
GACTTAA106700.028.609834
GCCTAAG129000.027.0333429
GTGCCTA128900.026.9890297
CGCAATC62650.026.4500122
AGACAAT157150.023.9382611
CCGCAAT76550.023.7242131
GCGATCT119950.023.4690063
GATCAAC197050.023.3473875
TGCCTAA151050.023.0790398
Produced by FastQC (version 0.11.5)