FastQCFastQC Report
mer. 11 mai 2022
SRR12596366_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12596366_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19997056
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGT276170.13810532910444417No Hit
CTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGA254160.12709870892995448No Hit
AAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAG245030.12253303686302623No Hit
GCCAGATCAACAAGATTTCATGGCGTTTCCTCTGTGGGAGATGCTGTGGA240870.1204527306419505No Hit
GAAAAAGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGT239030.11953259519801315No Hit
CTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGTGCCATCA229870.11495192092275983No Hit
AGACAATCTAACTTGAGAAAAAGAAAACGCAGCAATGAGTCTCTCTGATA225500.11276659924340862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCTA97200.047.7743384
CCGCGAT59800.044.0696681
ATCTAAC100400.038.434526
TCTAACT105100.035.8247227
CTAACTT115650.032.930258
AATCTAA125550.032.857855
CTTAAAC193750.032.443851
GACAATC130700.031.3430442
GGACTTA103400.031.1931113
CGCGATC88200.030.038882
TAACTTG129850.028.9409969
GACTTAA112150.028.8236054
GTGCCTA134300.027.713957
GCCTAAG134550.027.6625239
GCGATCT112200.024.6399363
AGACAAT161150.023.9940681
ACAATCT220150.023.7748053
TGCCTAA159150.023.6128738
CGCAATC58450.023.403112
TAAACAA264850.022.3446253
Produced by FastQC (version 0.11.5)