FastQCFastQC Report
mer. 11 mai 2022
SRR12596367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12596367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25544345
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAAGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGT997650.3905561093854628No Hit
GGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGT957810.37495970243120347No Hit
AGACAATCTAACTTGAGAAAAAGAAAACGCAGCAATGAGTCTCTCTGATA932040.3648713638967842No Hit
AAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAG803930.31471936352253305No Hit
AGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAG787820.3084126839032279No Hit
CTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGA779100.3049990125015928No Hit
GAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGC705260.276092418889582No Hit
CTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGTGCCATCA647210.2533672325518623No Hit
CTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGTTCGCTCA584080.22865334773704316No Hit
GTCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCAT563910.2207572752403712No Hit
TCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCATA549250.2150182359344113No Hit
CGAGGATCTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGT548930.2148929635893972No Hit
CAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGCCCTATGG482950.18906337195179598No Hit
CCACGATCACATTGTGTGCGAGGATCTTGAAGTTGGCCGGGTCAATACGC463480.18144133270984242No Hit
CCGCGATCTTCTGCAATCCAGCTGGTGTTCATCGGTGATGGGAATCTAGT413350.16181663691122242No Hit
GCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGCCCTATG389870.15262477859581053No Hit
GTCAATACGCAGCTTGAAAGCATGAAGTTCGCTCAGGGAGGACAGAGCTC384210.15040902399337308No Hit
CTGGACTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGA383900.15028766640914065No Hit
CAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATG373270.1461262756982025No Hit
CTTAAACAAGTGCATAAGGACACCCTAAAACGTAGACATGGTTGAGTGGA357270.1398626584474959No Hit
CACGATCACATTGTGTGCGAGGATCTTGAAGTTGGCCGGGTCAATACGCA336650.13179042171564784No Hit
CTCAATCCCGATGAACTCGGACCTCAGGCCCTGGCCAGGTGTCTGATCGT327550.12822798940430846No Hit
CTTGATGTTATCCATGTTCTTGATGGCTCTCTCCAGACCTCCCATCACAG326850.12795395614959007No Hit
GCCAGATCAACAAGATTTCATGGCGTTTCCTCTGTGGGAGATGCTGTGGA323960.12682259028368117No Hit
GTGGCCTTGATGTTATCCATGTTCTTGATGGCTCTCTCCAGACCTCCCAT304590.11923969864954455No Hit
TGGGAATCTAGTGGTACTGTCTGCACAGAGCGGAAACGACGACACACAGA302300.11834321843053716No Hit
CTCGGACCTCAGGCCCTGGCCAGGTGTCTGATCGTGTATCCCTGGACTCA287580.11258069055988713No Hit
AAACAAGTGCATAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAG277710.1087168216683575No Hit
CCGATGACCACGATCACATTGTGTGCGAGGATCTTGAAGTTGGCCGGGTC272800.10679467412454695No Hit
CGCAGCTTGAAAGCATGAAGTTCGCTCAGGGAGGACAGAGCTCCCACAAG259730.10167808178287602No Hit
GAGAAAAAGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTT256910.10057411924243899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCTA192500.075.6610644
ATCTAAC206650.068.278376
TCTAACT223750.062.6095667
GACAATC230050.062.2053032
AATCTAA234450.061.078875
GGACTTA167300.058.3551983
CCGCGAT107500.058.1312331
AGACAAT258350.054.626471
GACTTAA182400.053.641624
CTAACTT264700.053.576698
CTTAAAC343500.052.793781
TAACTTG281600.050.5403259
ACAATCT324500.046.3932843
CGCAATC95300.041.7365572
CGCGATC151750.041.690422
CCGCAAT100900.040.8605081
TAAACAA435300.040.6049163
TTAAACA473200.038.251222
ACTTAAA239750.036.7560845
GCGATCT181550.035.175733
Produced by FastQC (version 0.11.5)