FastQCFastQC Report
mer. 11 mai 2022
SRR12596367_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12596367_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25544345
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGT972540.3807261450626352No Hit
AGACAATCTAACTTGAGAAAAAGAAAACGCAGCAATGAGTCTCTCTGATA930310.3641941102815515No Hit
GAAAAAGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGT922020.3609487735935292No Hit
CTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGA890360.3485546409586936No Hit
AAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAG868710.3400791838663313No Hit
CTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGTGCCATCA757190.29642177162890654No Hit
AGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAG738250.2890072147083826No Hit
CTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGTTCGCTCA661640.2590162323598432No Hit
GAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGC646420.25305796645010864No Hit
CGAGGATCTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGT578440.22644542265616913No Hit
TCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCATA510680.1999190035994268No Hit
GTCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCAT502030.19653273552326356No Hit
CAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGCCCTATGG486640.1905079186802402No Hit
CCACGATCACATTGTGTGCGAGGATCTTGAAGTTGGCCGGGTCAATACGC479030.18752878572537288No Hit
CTGGACTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGA420420.16458437278387839No Hit
CAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATG414190.16214547681688452No Hit
CTTAAACAAGTGCATAAGGACACCCTAAAACGTAGACATGGTTGAGTGGA413220.16176574502106042No Hit
CCGCGATCTTCTGCAATCCAGCTGGTGTTCATCGGTGATGGGAATCTAGT385310.15083964767935917No Hit
CACGATCACATTGTGTGCGAGGATCTTGAAGTTGGCCGGGTCAATACGCA360810.14124848376421473No Hit
GTCAATACGCAGCTTGAAAGCATGAAGTTCGCTCAGGGAGGACAGAGCTC359690.14081003055666527No Hit
GCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGCCCTATG350180.13708709305327657No Hit
CTTGATGTTATCCATGTTCTTGATGGCTCTCTCCAGACCTCCCATCACAG337880.1322719372917959No Hit
CTCAATCCCGATGAACTCGGACCTCAGGCCCTGGCCAGGTGTCTGATCGT335660.13140286039826035No Hit
GCCAGATCAACAAGATTTCATGGCGTTTCCTCTGTGGGAGATGCTGTGGA308650.12082909152691135No Hit
TGGGAATCTAGTGGTACTGTCTGCACAGAGCGGAAACGACGACACACAGA300700.11771685670546651No Hit
AAACAAGTGCATAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAG298570.1168830126589662No Hit
CTCGGACCTCAGGCCCTGGCCAGGTGTCTGATCGTGTATCCCTGGACTCA290910.11388430590019043No Hit
GTGGCCTTGATGTTATCCATGTTCTTGATGGCTCTCTCCAGACCTCCCAT277020.10844670317442079No Hit
CCGATGACCACGATCACATTGTGTGCGAGGATCTTGAAGTTGGCCGGGTC274760.10756196723775849No Hit
CGCAGCTTGAAAGCATGAAGTTCGCTCAGGGAGGACAGAGCTCCCACAAG258230.10109086766562228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCTA200350.077.116974
ATCTAAC213650.069.8207556
TCTAACT227250.065.009037
GACAATC236300.063.983582
AATCTAA242250.063.3031585
GGACTTA173350.061.9661563
CCGCGAT99250.057.6759341
GACTTAA192600.056.3334774
AGACAAT262450.056.3248981
CTAACTT272550.055.3134468
CTTAAAC364900.054.5211561
TAACTTG287000.052.2776459
ACAATCT337300.047.1934783
TAAACAA462150.041.9997373
CGCAATC91850.041.8576742
CGCGATC147350.039.77292
TTAAACA498750.039.740772
CCGCAAT102400.039.6585851
ACTTAAA251250.038.3406645
ACAAGTG553350.034.6993066
Produced by FastQC (version 0.11.5)