FastQCFastQC Report
mer. 11 mai 2022
SRR12596368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12596368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21661960
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGATCAACAAGATTTCATGGCGTTTCCTCTGTGGGAGATGCTGTGGA918310.42392747470681325No Hit
AAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAG690790.3188954277452271No Hit
GAAAAAGAAGACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGT571080.2636326537395508No Hit
CTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGA521640.24080923425211753No Hit
GTCGGTTTTTGTATAATTTGTATAATAGAGGCTTGAGGCTTTGCGCGCGG476380.2199154647132577No Hit
GGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGT461970.21326325041685976No Hit
TCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCATA444050.20499068413015256No Hit
AGACAATCTAACTTGAGAAAAAGAAGACGCAGCAATGAGTCTCTCTGATA397800.1836398922350517No Hit
GTCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCAT349980.16156432751237654No Hit
GCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGCCCTATG334100.15423350426277216No Hit
CAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGCCCTATGG320480.14794598457387975No Hit
GATCAACAAGATTTCATGGCGTTTCCTCTGTGGGAGATGCTGTGGAAACT290100.13392139954094645No Hit
CTGGACTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGA285880.13197328404262587No Hit
CTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGTGCCATCA278710.12866333425045562No Hit
CGAGGATCTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGT276690.12773082398822636No Hit
CAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATG274450.12669675320238796No Hit
CTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGTTCGCTCA271930.12553342356831976No Hit
CCACGATCACATTGTGTGCGAGGATCTTGAAGTTGGCCGGGTCAATACGC255470.11793484984738223No Hit
GTCAATACGCAGCTTGAAAGCATGAAGTTCGCTCAGGGAGGACAGAGCTC241120.11131033387560497No Hit
CAAGATTTCATGGCGTTTCCTCTGTGGGAGATGCTGTGGAAACTTGACTG239400.11051631523647906No Hit
GTTTTTGTATAATTTGTATAATAGAGGCTTGAGGCTTTGCGCGCGGGATT234490.10824966900502078No Hit
GAAAAAGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGT227980.10524440078367792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCTA143900.066.449784
ATCTAAC155050.056.9328586
TCTAACT169500.051.3571477
AATCTAA184650.049.40615
GACAATC183300.049.2399252
GCCAGAT302700.049.235441
CTAACTT190000.046.57398
GGACTTA110000.046.412173
CCGCAAT85700.044.799811
GATCAAC318350.044.308085
TAACTTG200900.044.118679
AGACAAT208650.041.8076251
GACTTAA124700.041.6895374
CGCAATC93000.040.8184432
AGATCAA394200.038.0842824
CCGCGAT51650.037.5155831
GTGCCTA290400.036.5712437
ACAATCT278500.035.965023
CTTAAAC225050.035.5602531
GCCTAAG305100.034.6912049
Produced by FastQC (version 0.11.5)