FastQCFastQC Report
mer. 11 mai 2022
SRR12596368_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12596368_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21661960
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGATCAACAAGATTTCATGGCGTTTCCTCTGTGGGAGATGCTGTGGA837580.3866593789296998No Hit
CTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGA764940.35312594058894026No Hit
AAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAG705010.32545993068032625No Hit
GAAAAAGAAGACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGT486920.2247811370716223No Hit
GTCGGTTTTTGTATAATTTGTATAATAGAGGCTTGAGGCTTTGCGCGCGG432260.19954796334219066No Hit
GGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGT426740.19699971747708886No Hit
TCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCATA394050.18190874694625972No Hit
CTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGTGCCATCA391730.18083774506092706No Hit
AGACAATCTAACTTGAGAAAAAGAAGACGCAGCAATGAGTCTCTCTGATA381110.17593514160306822No Hit
CTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGTTCGCTCA368210.16998000180962386No Hit
CAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATG351840.16242297557561733No Hit
CAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGCCCTATGG351740.16237681170124957No Hit
CTGGACTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGA347120.16024404070545786No Hit
CAAGATTTCATGGCGTTTCCTCTGTGGGAGATGCTGTGGAAACTTGACTG329010.15188376305745188No Hit
CGAGGATCTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGT314830.14533772567209985No Hit
GTCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCAT291650.13463693959364711No Hit
GATCAACAAGATTTCATGGCGTTTCCTCTGTGGGAGATGCTGTGGAAACT281020.12972971974835149No Hit
CCACGATCACATTGTGTGCGAGGATCTTGAAGTTGGCCGGGTCAATACGC279640.12909265828207603No Hit
GCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGCCCTATG277990.12833095435500758No Hit
CACGATCACATTGTGTGCGAGGATCTTGAAGTTGGCCGGGTCAATACGCA224400.1035917340813112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCTA149300.062.4980474
ATCTAAC157950.055.1096846
GGACTTA117550.052.3678443
TCTAACT169550.050.7447747
AATCTAA186850.048.782255
GACAATC184100.048.0647132
GACTTAA132500.047.2742464
CTTAAAC261900.046.8509981
GCCAGAT302050.045.734081
CTAACTT194500.044.716588
GATCAAC315850.041.988465
CCGCAAT87600.041.749751
AGACAAT207900.041.706141
TAACTTG208600.041.659519
CGCAATC92050.039.5781022
CCGCGAT52650.038.6367651
AGATCAA406650.034.6136324
ACAATCT286550.034.0957833
TAAACAA352400.033.588233
CCAGATC429000.032.3241582
Produced by FastQC (version 0.11.5)