FastQCFastQC Report
mer. 11 mai 2022
SRR12596369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12596369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20562543
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGT618780.30092581447732414No Hit
GAAAAAGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGT487000.23683841050204735No Hit
AAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAG451140.21939893329341612No Hit
AGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAG434920.21151080389230068No Hit
GAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGC392560.1909102390691657No Hit
CTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGA364720.17737105765566058No Hit
TCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCATA340370.16552913713055822No Hit
GCCAGATCAACAAGATTTCATGGCGTTTCCTCTGTGGGAGATGCTGTGGA338850.1647899289499358No Hit
CTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGTGCCATCA317730.15451882580865606No Hit
GTCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCAT316390.1538671554388968No Hit
AGACAATCTAACTTGAGAAAAAGAAAACGCAGCAATGAGTCTCTCTGATA310630.15106594549127508No Hit
CTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGTTCGCTCA275470.13396689310266732No Hit
CGAGGATCTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGT243820.11857482802589153No Hit
GTCAATACGCAGCTTGAAAGCATGAAGTTCGCTCAGGGAGGACAGAGCTC220350.10716087013167583No Hit
CCACGATCACATTGTGTGCGAGGATCTTGAAGTTGGCCGGGTCAATACGC218330.10617850136532238No Hit
CAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGCCCTATGG211540.10287638061109465No Hit
CAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATG206650.10049827008264493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCTA86350.065.8686754
ATCTAAC90450.054.843466
GGACTTA92450.054.4364433
AATCTAA92300.053.822225
GACTTAA102150.049.7600174
CCGCGAT75900.040.6079671
CTTAAAC211700.039.3909951
CTAACTT123650.039.3073238
TCTAACT130700.036.9658057
TAACTTG135550.036.2815679
AGACAAT131800.036.2795521
TAAACAA220850.036.121173
GTGCCTA192400.035.1409767
GCCTAAG198100.034.203469
GACAATC155200.032.1979642
CGCGATC96700.031.2742
CGCAATC62650.031.1464732
ACTTAAA150400.030.6848775
AACAAGT317850.030.423872
TTAAACA277300.029.9651572
Produced by FastQC (version 0.11.5)