FastQCFastQC Report
mer. 11 mai 2022
SRR12596369_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12596369_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20562543
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGT616900.3000115306749753No Hit
AAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAG483110.2349466211450597No Hit
GAAAAAGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGT462110.22473387654435542No Hit
CTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGA458810.22312901667853047No Hit
CTAAAACGTAGACATGGTTGAGTGGACAGATGCTGAGCGAAGTGCCATCA407590.1982196462762412No Hit
AGAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAG405070.19699411692415672No Hit
GAAAACGCAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGC362290.17618929720900767No Hit
AGACAATCTAACTTGAGAAAAAGAAAACGCAGCAATGAGTCTCTCTGATA342830.166725487212355No Hit
GCCAGATCAACAAGATTTCATGGCGTTTCCTCTGTGGGAGATGCTGTGGA318600.1549419252278281No Hit
CTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGTTCGCTCA318110.15470362785381164No Hit
TCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCATA301200.1464799368443874No Hit
GTCGGGATCCACATGCAATTTCTCAGAGTGCATCACACTGAGTGGCGCAT267670.13017358796526285No Hit
CGAGGATCTTGAAGTTGGCCGGGTCAATACGCAGCTTGAAAGCATGAAGT253030.12305384601505759No Hit
CAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGAGTGGACAGATG250610.12187694878011926No Hit
CAGCAATGAGTCTCTCTGATAAGGACAAGGCTGTTGTGAAAGCCCTATGG224450.10915478693467048No Hit
CCACGATCACATTGTGTGCGAGGATCTTGAAGTTGGCCGGGTCAATACGC223950.10891162634893943No Hit
CTGGACTTAAACAAGTGCCTAAGGACACCCTAAAACGTAGACATGGTTGA217760.10590129829758897No Hit
CTTAAACAAGTGCATAAGGACACCCTAAAACGTAGACATGGTTGAGTGGA216390.10523503829268586No Hit
CTTTGGTGTTCCTGGTGCTCCTTGGAGCTGCCTTTGCTCTGGATGATGAC206660.10050313329435956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCTA96300.068.6394654
GGACTTA99400.059.9067273
ATCTAAC99750.056.160866
AATCTAA105100.054.7401285
GACTTAA110300.054.3131944
CTTAAAC237300.045.363141
CTAACTT136850.041.3018728
TAAACAA252600.041.0473983
AGACAAT138300.040.3495941
TCTAACT139550.039.9871987
TAACTTG145200.038.480349
CCGCGAT73850.036.952891
GACAATC162550.035.968942
TTAAACA299450.035.6595152
ACTTAAA156300.035.1040955
GTACCTA49250.034.6497154
TACCTAA52150.033.4135675
GTGCCTA215300.033.342667
GCCTAAG220500.032.5232969
ACCTAAG64000.030.7149436
Produced by FastQC (version 0.11.5)