FastQCFastQC Report
ven. 13 mai 2022
SRR2045415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2045415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22436517
Sequences flagged as poor quality0
Sequence length100
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1105760.4928394188812818TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA300500.054.6670152
GATCGGA322800.051.6299861
TCGGAAG317850.051.6006163
CGGAAGA364750.044.753824
AGAGCAC381800.042.903578
GAGCACA395000.041.184229
TATGCCG222400.037.02902646-47
AAGAGCA441900.036.8658037
CTCGTAT234500.035.2193442-43
CGTATGC263950.034.68844-45
TCGTATG241050.032.67451542-43
GAAGAGC509250.032.1685566
AGTGATC256350.031.22017536-37
GTATGCC246900.029.10735544-45
ATCTCGT281200.029.05090340-41
TCTCGTA261800.028.93593640-41
GGAAGAG584700.028.5651825
ATGCCGT271600.028.05679946-47
CACACGT317250.027.79310212-13
AGTCACA302900.027.68480528-29
Produced by FastQC (version 0.11.5)