FastQCFastQC Report
ven. 13 mai 2022
SRR2045415_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2045415_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22436517
Sequences flagged as poor quality0
Sequence length100
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG479080.2135269034850641Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA242850.045.1472552
TCGGAAG263350.041.4641233
GATCGGA269900.040.8017651
AAGAGCG261700.039.8436477
GAGCGTC239750.039.04969
AGAGCGT245350.038.4451568
CGGAAGA314400.035.0453034
GTATCAT153450.030.08816750-51
TCGTGTA164550.029.87074914-15
CGCCGTA168350.028.68825146-47
ATCATTA182300.028.09937152-53
CCGTATC177150.027.59664548-49
CGTATCA141500.026.99538248-49
AGCGTCG212750.026.16896610-11
GCCGTAT156950.026.10005846-47
TAGATCT227400.025.2053532-33
GTAGGGA193700.024.68496718-19
GATCTCG268050.023.9968234-35
GAAGAGC461950.023.2217796
TCTCGGT232950.023.06695436-37
Produced by FastQC (version 0.11.5)