FastQCFastQC Report
ven. 13 mai 2022
SRR2045417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2045417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35480430
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1938840.5464533547084971TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA812050.035.441944
TCGGAAG800750.035.243923
ATCGGAA812250.034.859982
GAGCACA815700.034.8312079
AGAGCAC857500.033.4742558
TATGCCG463450.032.09952546-47
GATCGGA895500.031.91841
CTCGTAT470700.030.80726242-43
GAAGAGC936700.030.4728016
AAGAGCA964750.029.5325367
CGTATGC510450.029.47765544-45
ATCTCGT509050.027.4122340-41
GGAAGAG1077350.026.9848885
AATCTCG459150.026.51392438-39
CACGTCT618350.026.35502214-15
ACTGACC612200.026.32758932-33
AGTCACT616150.026.02220728-29
CAATCTC548450.025.91204838-39
TCGTATG507350.025.70602642-43
CGTCTGA645500.025.44232616-17
Produced by FastQC (version 0.11.5)