FastQCFastQC Report
ven. 13 mai 2022
SRR13996120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13996120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22503120
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCTCGTAT5976202.655720628961673TruSeq Adapter, Index 8 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCGCGTAT2388521.0614172612508843TruSeq Adapter, Index 8 (97% over 38bp)
CATCTCTAAATCTAAATCTATTAAGCCATGGCCAACTTAACTCCTCTCCT225650.10027498409109492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1590600.084.609169
TCGGAAG1609750.084.586723
AGAGCAC1614600.083.6082848
GATCGGA1666650.081.869911
ATCGGAA1670600.081.5444642
GAAGAGC1785550.076.092356
CGGAAGA1813400.075.103284
GGAAGAG1846950.073.7968145
AAGAGCA1847050.073.3746957
GGCCGAT169350.033.4351431
GCCGATT185550.029.3441452
CCGATTG189650.029.1608683
CGATTGA202450.028.9177744
ATCTCTA218700.027.5966192
CGGGGAT87900.026.225691
TTAGTAT170050.026.1242987
CTCTAAA245750.024.9067754
AAGGGGG985750.024.86918365-69
AAAGGGG988950.024.5542865-69
TATGCCG1101500.024.29465745-49
Produced by FastQC (version 0.11.5)