FastQCFastQC Report
lun. 3 janv. 2022
SRR15718795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15718795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21802822
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCTCGTAT2701491.2390552012028535TruSeq Adapter, Index 8 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCGCGTAT1211470.5556482550745037TruSeq Adapter, Index 8 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG1033050.062.712443
GAGCACA1049000.061.352069
ATCGGAA1075450.060.320252
GATCGGA1100550.059.128081
AGAGCAC1091200.059.032178
CGGAAGA1123700.057.7511564
GAAGAGC1274000.050.878376
GGAAGAG1297350.050.112475
AAGAGCA1323400.048.9737327
CGTATGC566600.021.8559445-49
TATGCCG577050.021.84937545-49
AAGGGGG458150.021.14133865-69
GTATGCC604300.020.7711945-49
CGCGTAT198550.020.56522640-44
CGCTAAT625050.020.56076235-39
TCGCTAA636750.020.10382835-39
CCGTCTT611200.019.97840550-54
TCGCGTA208450.019.9269640-44
ATGCCGT635700.019.61158445-49
GCTAATC662200.019.55078735-39
Produced by FastQC (version 0.11.5)