FastQCFastQC Report
lun. 3 janv. 2022
SRR15718795_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15718795_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21802822
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG273940.12564428586354554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTG175850.017.853023
GGCCGAT164500.017.5567321
CGATTGA209600.016.6605434
GCCGATT206550.015.1327822
GGATAAT326800.014.0605991
AATCTAA210100.014.0163825
CCCGGGT43650.013.5299061
TTAAGCC196700.013.4700187
GTCGATT325200.013.4653691
CTAAATC258600.012.5882162
CGGGGAT91700.012.5665451
ATAATGT341200.012.55673
TAATGTT356650.012.5166514
ATTAAGC249700.012.4563546
ATCTAAA237000.012.2124686
TTCAACG301500.012.1072467
CTATTAA157950.011.9431914
TAAGCCA219800.011.9233558
GATAATG367400.011.840012
ATGTTGC404200.011.8276246
Produced by FastQC (version 0.11.5)