FastQCFastQC Report
lun. 3 janv. 2022
SRR15718796_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15718796_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20169850
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGGCAATCTCGTAT1258240.6238221900509919TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGGCAATCGCGTAT331200.16420548491932266TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGGCAATCTCGTTT323250.1602639583338498TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC810650.049.498878
GAGCACA821750.048.707619
ATCGGAA847250.047.8372152
GATCGGA850400.047.668851
TCGGAAG852850.047.4906853
CGGAAGA965200.041.8399854
GAAGAGC985500.041.0745856
AAGAGCA1050250.038.562827
GGAAGAG1164000.034.9860535
AAGGGGG328050.018.71835965-69
TATGCCG225250.017.4753545-49
AGGGGGG316300.016.66700665-69
CGTATGC238450.016.52607245-49
AAAGGGG388000.015.59595365-69
CCGTCTT298050.015.54983450-54
TCGTATG203450.015.35656945-49
TCTCGTA281850.015.08982440-44
CTCGTAT272800.015.08903740-44
TGCCGTC293550.014.81217150-54
GCCGTCT267500.014.79601750-54
Produced by FastQC (version 0.11.5)