FastQCFastQC Report
lun. 3 janv. 2022
SRR15718799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15718799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18291366
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTGAGCATCTCGTAT1077870.5892780233034536TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTGAGCATCGCGTAT466830.25521877370995694TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG565050.044.9790043
AGAGCAC573200.044.0682568
GATCGGA592500.043.121081
CGGAAGA591100.042.8832054
GAGCACA598300.042.2194949
ATCGGAA619200.041.0747342
AAGAGCA770050.033.0179757
GAAGAGC770450.033.000836
GGAAGAG850450.030.0827355
GTCACGT47950.019.9961911
TATGCCG257250.019.18521545-49
AGGGGGG220800.019.01570365-69
CGTATGC260500.018.6197845-49
ATGCCGT273550.017.67887345-49
AAGGGGG239150.017.66507365-69
GCCGTCT284500.016.70532850-54
CACGTAT67850.016.6814611
TGCCGTC284000.016.35958750-54
TCACGTA60350.016.0068042
TCGTATG200050.015.86186845-49
Produced by FastQC (version 0.11.5)